Azure II (CAS: 37247-10-2)

Abstract

A version of Mallory’s phloxine-methylene blue-azure II technique suitable for large epoxy sections is described. Phloxine B (C.I. 45410) and a yellow-green interference filter (546-548 nm transmission) combine to give high contrast monochrome images. By comparing light micrographs of lung parenchyma entirely unstained or stained only with phloxine B against electron micrographs of the same material, it is seen that phloxine B emphasizes essentially only elastin and collagen fiber bundles. The technique has produced images useful for investigating lung parenchyma architecture and micromechanics.

Appearance description

Dark green powder, methylene blue and azure I equivalent mixtures; Dark blue is dissolved in water, slightly soluble in ethanol, slightly soluble in chloroform, insoluble in ether

Application

Biological staining; Blood stain

Storage transport

Sealed storage 

Hazard statements 

Dangerous code: Xn

Level of danger: 22-41

Level of security: 26-39